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Because of the advantages of high safety, strong stability and low cost, recombinant protein vaccine has been widely used in the prevention of viral hepatitis B (hepatitis B), pertussis, diphtheria, tetanus (DPT), influenza (influenza) and other infectious diseases. Recombinant protein vaccines mainly include recombinant subunit vaccine, VLP vaccine, etc., and the expression system includes Escherichia coli, yeast, insect cells and mammalian cells. The expression system should be selected according to the characteristics of protein antigens. The insect cells commonly used to culture baculovirus expression were Sf 9 cells, Sf 21 cells, which were cultured in bioreactors using serum-free medium. Mammalian cells HEK293, CHO, etc., commonly used for preparing recombinant protein vaccines, were cultured in bioreactors using serum-free media. A number of bioreactor devices can be used in the production of recombinant vaccines, helping biomedical companies to easily adjust production capacity and support the production of traditional vaccines and emerging vaccines using recombinant technologies.
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